Metformin Promotes Apoptosis İn Primary Breast Cancer Cells By Downregulation Of Cyclin D1 And Upregulation Of P53 Through An Ampk-Alpha İndependent Mechanism

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dc.authorid303337en_US
dc.authorid262746en_US
dc.authorid252983en_US
dc.contributor.authorYenmiş, Güven
dc.contributor.authorBeşli, Nail
dc.contributor.authorSaraç, Elif Yaprak
dc.contributor.authorEmre, Fatma Sinem Hocaoğlu
dc.contributor.authorŞenol, Kazım
dc.contributor.authorKanıgür Sultuybek, Gönül
dc.date.accessioned2021-09-09T12:07:25Z
dc.date.available2021-09-09T12:07:25Z
dc.date.issued2021
dc.departmentİstanbul Beykent Üniversitesien_US
dc.description.abstractBackground/aim: In the present study we aimed to figure out the effect of metformin on the expression of AMPK-alpha, cyclin D1, and Tp53, and apoptosis in primary breast cancer cells (PBCCs). Materials and methods: PBCCs were treated with two doses of metformin (0 mM, 25 mM). Proliferation was determined by BrdU as- say. Real-time PCR was used to assess AMPK-alpha, cyclin D1, and Tp53 gene expressions; apoptotic indexes of PBCCs were analyzed using flow-cytometry. Results: Twenty-four–hour incubation with 25 mM metformin reduced the proliferation of PBCCs. AMPK-alpha gene expression in PBCCs was not affected by 25 mM metformin treatment compared with the control group. PBCCs treated with 25 mM metformin had lower cyclin D1 expression compared with nontreated cells; however, the difference was not statistically significant. Twenty-five mil- limolar dose of metformin increased p53 expression significantly compared with the nontreated group. The high concentration of met- formin elevated the number of annexin V-positive apoptotic cells, and the increase in the apoptotic index was statistically significant. Conclusion: Metformin can modulate cyclin D1 and p53 expression through AMPK-alpha-independent mechanism in breast cancer cells, leading to cell proliferation inhibition and apoptosis induction.en_US
dc.identifier.citationTurk J Med Sci (2021) 51: 826-834en_US
dc.identifier.doi10.3906/sag-1908-112
dc.identifier.issn2149-6498
dc.identifier.pmid33350292en_US
dc.identifier.scopus2-s2.0-85102106751en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.trdizinid484958en_US
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/484958
dc.identifier.urihttps://doi.org/10.3906/sag-1908-112
dc.identifier.wosWOS:000646231300004en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTUBİTAKen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.subjectAMPK-alphaen_US
dc.subjectTp53en_US
dc.subjectMetforminen_US
dc.subjectCyclin D1en_US
dc.subjectBreast cancer.en_US
dc.titleMetformin Promotes Apoptosis İn Primary Breast Cancer Cells By Downregulation Of Cyclin D1 And Upregulation Of P53 Through An Ampk-Alpha İndependent Mechanismen_US
dc.typeArticleen_US

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