Effects of Parasitism by Endoparasitoid Pimpla Turionellae on Hemolymph Proteins of Hosts Galleria Mellonella

Özet

The plasma proteins were determined under the effect of parasitism by endoparasitoid, Pimpla turionellae (Hymenoptera: Ichneumonidae) on hemolymph proteins of host, Galleria mellonella (Lepidoptera: Pyralidae) by the colorimetric and SDS-PAGE (Sodium dodecyl sulfate- polyacrylamide gel electrophoresis) methods. In the colorimetric analysis it was showed a decrease in the total plasma protein levels in parasitized host during the first hour compared with unparasitized controls whereas increased total plasma protein levels were determined during the third hour. However, In the SDS-PAGE analysis it revealed that the levels of 15.5, 17.5, 67 and 82 kDa proteins decreased in parasitized host, compared to unparasitized controls. After 72 h of treatment, plasma protein in the parasitized host was different to that of unparasitized controls. In conclusion, because of parasitization protein levels were found as a different in the colorimetric and the electrophoresis analysis.

Açıklama

Anahtar Kelimeler

Hemolymph protein, SDS-PAGE, parasitization, Pimpla turionellae, Galleria mellonella

Kaynak

WoS Q Değeri

Scopus Q Değeri

Cilt

Sayı

Künye

Journal of Science and Technology 1 (1), 2007, 146-156

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