Salkin, Hasan2024-03-132024-03-1320232149-78932536-507Xhttps://doi.org/10.4274/cjms.2021.3236https://search.trdizin.gov.tr/yayin/detay/1178719https://hdl.handle.net/20.500.12662/3902BACKGROUND/AIMS: The aim of this study was to evaluate the effects of 10% platelet rich plasma (PRP) on the biological activity of human adipose-derived mesenchymal stem cells (hADSCs).MATERIALS AND METHODS: hADSCs were obtained from Erciyes University Gevher Nesibe Genom and Stem Cell Institute. The cells were transferred into plates at a density of 5,000 cells/cm2 in DMEM supplemented with 10% fetal bovine serum. Immunophenotyping was performed by means of primary antibodies selected against CD44, CD90, CD105, CD34, CD45 and CD73 antigens. The experimental groups were composed of 10% PRP treated cells (the study group) and untreated cells (the study group). 2,5-diphenyltetrazolium bromidetest was performed to demonstrate proliferation. Viability and apoptosis were evaluated in the experimental groups on days 1, 3 and 7. Senescent cells were determined by beta-galactosidase staining in the experimental groups on day 7.RESULTS: hADSCs were positive for the immunophenotype of mesenchymal stem cells. The viability and proliferation parameters were statistically significant and higher in the study group at days 1, 3 and 7. Apoptosis and senescence were lower in the 10% PRP group (p<0.05).CONCLUSION: 10% PRP increases proliferation and viability, and prevents the senescence and total apoptosis in hADSCs. In regenerative medical studies, 10% PRP can be used to increase the biological characteristics of hADSCs.eninfo:eu-repo/semantics/closedAccessPlatelet rich plasmaadipose-derived mesenchymal stem cellapoptosisproliferationcellular senescenceArticle10.4274/cjms.2021.3236146214211787198WOS:000992822000010N/A