Demirci, M.Yigin, A.Altun, S. K.Uysal, H. K.Saribas, S.Kocazeybek, B. S.2024-03-132024-03-1320191119-3077https://doi.org/10.4103/njcp.njcp_596_18https://hdl.handle.net/20.500.12662/3896Aims: The aim of this study was to provide epidemiological data about the presence of Salmonella spp. and Shigella spp. in raw milk samples collected from different animals. Methods: A total of 231 raw milk samples from 48 cows, 65 goats, 65 sheep, and 53 donkeys were studied. The ISO 6579:2002 and ISO 21567:2004 methods, antimicrobial susceptibility tests, and serotyping were performed. Species and subspecies discriminations were made via matrix-assisted laser desorption/ionization-time of flight mass spectrometry. After DNA isolation from all samples, Salmonella spp. and Shigella spp. were detected using real-time polymerase chain reaction (PCR) kits. Results: Five samples (2.16%) showed positivity out of 231 raw milk samples for Salmonella spp., and 2 (0.87%) samples were detected to be positive by multiplex real-time PCR design. Conclusion: We found that raw milk samples were not free of Salmonella spp. and Shigella spp. and need to be tested routinely to avoid public health problems. Rapid and reliable real-time PCR method can be developed and used for this purposes instead of slow bacterial culture processes.eninfo:eu-repo/semantics/closedAccessMALDI-TOF MSqPCRSalmonella sppShigella sppsubspecies discriminationArticle10.4103/njcp.njcp_596_182-s2.0-850709522081090831417051Q3108322WOS:000481990500008Q4