Karaboga, IhsanOkuyan, Hamza MalikPolat, Fatin RustuBicakci, NurcanDuran, YasinGunduz, IhsanBeyaz, Yuksel2026-01-312026-01-3120251567-23791567-2387https://doi.org./10.1007/s10735-025-10645-7https://hdl.handle.net/20.500.12662/10617Acute pancreatitis (AP) is a serious inflammatory condition of the pancreas often requiring hospitalisation and characterised by abdominal pain, nausea, and vomiting. Hyperin (HP), a natural flavonoid, possesses antioxidant and anti-inflammatory characteristics. However, the effect of HP on AP remains unclear. This study aimed to evaluate the protective effects of HP in a cerulein-induced AP model in rats, focusing on histopathological damage, inflammatory cytokines, and oxidative stress markers. All rats were randomly assigned into control (n = 8), AP (n = 8), and AP + HP (50 mg/kg) groups (n = 8). We created an AP rat model by intraperitoneal cerulein injections. We evaluated histopathologically pancreatic tissues using hematoxylin-eosin staining. NF-kappa B and TNF-alpha expressions were analysed using immunohistochemical method. Oxidative stress markers such as MDA, SOD, CAT, GPx as well as serum amylase and lipase levels were assessed using biochemical methods. IL-6 and IL-10 levels were measured using ELISA. HP treatment significantly reduced histological damage scores, including oedema, necrosis, and vacuolisation. Moreover, expression levels of NF-kappa B and TNF-alpha were markedly decreased in the AP + HP group. HP restored SOD activity and reduced MDA levels, indicating attenuated oxidative stress. HP decreased serum IL-6 and increased IL-10 levels, along with significant reductions in amylase and lipase. HP exerts both anti-inflammatory and antioxidant effects in cerulein-induced AP, primarily through NF-kappa B and TNF-alpha inhibition and SOD activation. These findings suggest that HP may be a promising natural compound for the adjunctive treatment of AP.eninfo:eu-repo/semantics/closedAccessHyperinAcute pancreatitisInflammationOxidative stressCeruleinArticle10.1007/s10735-025-10645-72-s2.0-105019536785641128823Q256WOS:001600493700001Q4