Uskur, TugceTasdemir, Fatma UnluOzturk, MehmetaykutSonmez, HaktanSeven, Zeynep Gizem TodurgaGokdemir, SelimBastan, Burak2026-01-312026-01-3120252475-05732475-0581https://doi.org./10.5152/pcp.2025.241005https://search.trdizin.gov.tr/tr/yayin/detay/1361095https://hdl.handle.net/20.500.12662/10841Background: This study aims to investigate the effects of vitamin B12 on morphine-induced conditioned place preference (CPP), a model commonly used to assess the rewarding effects of drugs. Morphine is a potent analgesic widely used for moderate to severe pain, but it also poses a significant risk of addiction. Previous studies suggest that cyanocobalamin (vitamin B12) may enhance the analgesic effects of morphine and reduce tolerance, but its impact on morphine addiction remains unclear. Methods: The experiment followed phases of habituation, pre-conditioning, conditioning, and post-conditioning. Adult male Wistar albino rats (250-300 g) were randomly divided into 3 groups (n = 8 per group): control (saline), morphine (10 mg/kg), and a combination group of vitamin B12 (2 mg/kg) with morphine. The effects of saline, morphine, and the morphine-vitamin B12 combination on CPP were assessed. All drugs and saline were administered intraperitoneally (ip). Results: Morphine (10 mg/kg) significantly induced CPP compared to the saline group (P < .0001). Vitamin B12 (2 mg/kg) did not produce a statistically significant difference in morphine-induced CPP compared to the control group. Conclusion: Morphine induces a significant place preference, and vitamin B12 did not produce a statistically significant difference in reducing this effect. Further research with different doses of vitamin B12 is necessary to fully investigate these effects.eninfo:eu-repo/semantics/openAccessArticle10.5152/pcp.2025.2410052-s2.0-105012591121274340824234Q4269136109535WOS:001548315400009Q4